Bacillus Subtilis

Bacillus subtilis is a Gram-positive, aerobic, spore-forming bacterium. After ingested, the spores of Bacillus subtilis will 

germinate, multiply, temporarily colonize in the upper digestive tract execute excellent positive effect.



Mode of action of GBW Bacillus Subtilis:


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① Secretion of digestion enzyme   ② Inhibition of pathogen adhesion and colonization

Production of antibacterial substances  Maintain intestinal integrity

Consumption of oxygen create anaerobic intestinal environment  Boost immunity



Main functions:


1. Secret Xylanase, protease, and amylase, increase the growth performance and improve feed conversion ratio


2. Favor the environment of Lactobacilli and other lactic acid producing bacteria by creating a positive anaerobic environment


3. Reducing the colonization of E.coli, Salmonella, Clostridium perfringens and other pathogen by Competitive exclusion and 

    secreting of bacteriocin, prevents enteric disease


4. Enhance immunity, reduce use of antibiotics and maintain animal health


5. Improve the quality of animal products such as meat, eggs, and milk


6. Secret enzyme that can degrade ammonia and H2S, reduce noxious gas emission 



GBW Bacillus subtilis has high survival rate


1. When being processed in the feed-milling procedure


2. In the highly acidic and bile upper gastro-intestinal tract


3. During enzymatic digestion


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GBW Bacillus subtilis was placed in a solution with a pH of 2.0 and a pepsin concentration of 0.32%, treated at 39 °C 

for different time to detect survival rate of Bacillus subtilis.


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Result analysis: after pelleting under 85, 0.45MPb, the survival rate of GBW Bacillus subtilis is above 92%.


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GBW Bacillus subtilis was placed in a solution with a pH of 2.0 and bile concentration of 4mg/L, treated at 39°C for 1 hour 

to detect survival rate of Bacillus subtilis.


High germination rate


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Sixty broiler in 30 day old were divided into four group, one group is supplement with 100 g (2 million CFU/g) GBW Bacillus 

subtilis, the other three group were supplement with 100 g (2 million CFU/g) Bacillus subtilis from other company. 1.5h after 

supplement, the Germination rate of Bacillus subtilis in duodenum were tested.


Excellent enzyme-secretion ability


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The enzyme activity of xylanase produced by the single colony cultivated in medium for 24h is 300 U/g.


Components: Bacillus subtilis, Carrier


Guaranteed value: 20 billion CFU/g


Usage and dosage: Add 100-150g (20 billion CFU/g) to per ton complete feed


Package and storage:

Keep in cool, dry and shady conditions and seal the bag when it’s not in use, and shelf life is 12 months in original package under 25.

25kg/bag.




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